Development of Two Antibody-Detection ELISAs for Serodiagnosis of Human Chronic
نویسندگان
چکیده
3 4 Kimberly Cabán-Hernández, José F. Gaudier, Caleb Ruiz-Jiménez, Ana M. Espino# 5 6 7 Laboratory of Immunology and Molecular Parasitology, Department of Microbiology, 8 University of Puerto Rico, School of Medicine 9 10 11 12 Running Head: ELISA for serodiagnosis of human fascioliasis 13 14 15 16 #Address correspondence to Espino, A.M. [email protected] 17 Present address: Laboratory of Immunology and Molecular Parasitology, Department of 18 Microbiology, University of Puerto Rico, School of Medicine. PO BOX 365067, San 19 Juan, PR 00936-5067 20 21 KCH and JFG contributed equally to this work 22 23 JCM Accepts, published online ahead of print on 18 December 2013 J. Clin. Microbiol. doi:10.1128/JCM.02875-13 Copyright © 2013, American Society for Microbiology. All Rights Reserved.
منابع مشابه
Development of two antibody detection enzyme-linked immunosorbent assays for serodiagnosis of human chronic fascioliasis.
Coprological examination based on egg detection in stool samples is currently used as the gold standard for the diagnosis of human fascioliasis. However, this method is not effective during the acute phase of the disease and has poor sensitivity during the chronic phase. Serodiagnosis has become an excellent alternative to coprological examination in efforts to combat the effects of fascioliasi...
متن کاملSimultaneous Detection of IgA/IgG AntiTissue Transglutaminase/Deamidated Gliadin Peptides in Serodiagnosis of Celiac Disease
Background: Celiac disease is a common autoimmune disorder that is diagnosed based on clinical case identification, serological screening, and duodenal histology. However, the existence of mild clinical forms, such as seronegative cases with patchy atrophy and potential celiac disease, can make it difficult to determine a definitive diagnosis. The seronegative patients with celiac disease can i...
متن کاملDevelopment of ELISAs for the detection of urogenital chlamydia trachomatis infection targeting the pORF5 protein.
OBJECTIVE To prepare antibodies against pORF5 plasmid protein of Chlamydia trachomatis and develop double-antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISAs) for the detection of genital C. trachomatis infections. METHODS The pORF5 protein was expressed in Escherichia coli and used to immunize BALB/c mice and New Zealand rabbits to produce monoclonal antibodies (mAbs) and polycl...
متن کاملStandardization of an Enzyme-Linked Immunosorbent Assay for Detection of Infectious Bronchitis Virus Antibody.
An indirect enzyme–linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis virus (IBV). Antigen was prepared from whole-purified IBV Massachusetts serotype (BR 801 strain). Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA was determined 0.1μg/ml, 1:3000 and 1:100, respe...
متن کاملEvaluation of Enzyme Linked Immunosorbent Assay, Utilizing Native Antigen B for Serodiagnosis of Human Hydatidosis
Background: Hydatidosis is one of the cosmopolitan parasitic zoonoses caused by the larval stage of Echinococcus granulosus. Diagnosis of hydatidosis is still an unresolved problem. Serological tests using crude antigens for diagnosis of E. granulosus are sensi-tive, however their specificity are not satisfactory. Therefore, WHO recommended spe-cific serological methods using specific antigens,...
متن کامل